Fig. 4

BBD activates AMPK pathway to regulate hepatic gene expression involved in lipid metabolism. a Concentration of Leptin in portal vein serum was detected by Leptin Elisa test kit. b Representative immunohistochemistry staining for leptin in liver tissues of each group. c The expression of hepatic genes (SREBP1-c, ACC, SCD-1, LXRα, CD36, CPT-1 and PPARα) involved in lipogenesis or β-oxidation in liver and adipose tissues of different groups. d Western blot was used to detect the expression of AMPK and p-AMPK in livers of each group. The data were shown as mean ± SD. n  =  7 for all grou ps. *p < 0.05,**p < 0.01