Fig. 4
Gm21284 acts as ceRNA to regulate expression of Lhx8 with miR-30e-3P. a The predicted target region of miRNAs on Lhx8 3′UTR. b Statistical representation of luciferase reporter gene assay for miR-30e-3P, *: vs. NC miRNA, P < 0.05; **: vs. GV268-lhx8 mut, P < 0.05. c Statistical graph of luciferase reporter gene assay for miR-431, *: vs. NC miRNA, P < 0.05; **: vs. GV268-lhx8 mut, P < 0.05. d Statistical graph of luciferase reporter gene assay for miR-147. e Relative Lhx8 expression in each group for rescue assay. f Statistical graph of rescue assay for miR-30e-3P, *: vs. NC LV-GM21284 + miR-30e-3P group, P < 0.05; **: vs. NC group, P < 0.05. g Statistical graph of rescue assay for miR-431, *: vs. NC LV-GM21284 + miR-431 group, P < 0.05; **: vs. NC group, P < 0.05. g RNAhybrid 2.2 predicted miRNA sponge sites of Gm21284. h Statistical graph of luciferase reporter gene assay for miR-30e-3P, *: vs. NC miRNA, P < 0.05; **: vs. GV268-lhx8 mut, P < 0.05. i Statistical graph of luciferase reporter gene assay for miR-431