Table 1 Mouse qRT-PCR primer sets
From: Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells
Gene | Forward sequence | Reverse sequence |
|---|---|---|
Oct4 | GTGGAGGAAGCCGACAACAATGA | CAAGCTGATTGGCGATGTGAG |
Sox2 | CAGGAGAACCCCAAGATGCACAA | AATCCGGGTGCTCCTTCATGTG |
Esrrb | CAGGCAAGGATGACAGACG | GAGACAGCACGAAGGACTGC |
Klf4 | GTGCAGCTTGCAGCAGTAAC | AGCGAGTTGGAAAGGATAAAGTC |
Nanog | TGGTCCCCACAGTTTGCCTAGTTC | CAGGTCTTCAGAGGAAGGGCGA |
Cdx2 | AGGCTGAGCCATGAGGAGTA | CGAGGTCCATAATTCCACTCA |
Gata6 | CTCAGGGGTAGGGGCATCA | GAGGACAGACTGACACCTATGTA |
Foxa2 | CCCTACGCCAACATGAACTCG | GTTCTGCCGGTAGAAAGGGA |
Fgf5 | CTGTATGGACCCACAGGGAGTAAC | ATTAAGCTCCTGGGTCGCAAG |
Nestin | GCAGGGTCTACAGAGTCAGATCG | CAGCAGAGTCCTGTATGTAGCCA |
Gata2 | GCCTGTGGCCTCTACTACAAGC | CCCTTTCTTGCTCTTCTTGGAT |
Hand1 | AGGATGCACAAGCAGGTGA | GAGGCAGGAGGGAAGCTTT |
Brachyury | GCTTCAAGGAGCTAACTAACGAG | CCAGCAAGAAAGAGTACATGGC |
Gapdh | AGAGACGGCCGCATCTTCTTG | TGAAGGGGTCGTTGATGGCA |