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Fig. 5 | Cell & Bioscience

Fig. 5

From: Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells

Fig. 5

Photoactivation inhibits the self-renewal of ChR2-GFP-V6.5 ESCs. a Brightfield image of photoactivated and nonphotoactivated ChR2-GFP-V6.5 ESCs. b qRT-PCR analysis of the TFs Oct4, Sox2, Nanog, Esrrb and Klf4 in photoactivated and nonphotoactivated ChR2-GFP-V6.5 ESCs. c Colony formation assay of photoactivated and nonphotoactivated ChR2-GFP-V6.5 ESCs. d Statistical analysis of the colony formation data of photoactivated and nonphotoactivated ChR2-GFP-V6.5 ESCs. e Immunostaining and semi-quantification for the pluripotency markers OCT4 and SSEA-1 in photoactivated and nonphotoactivated ChR2-GFP-V6.5 ESCs. (n = 6, ***P < 0.001 compared to the nonphotoactivated control)

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