Fig. 3
From: Optogenetic stimulation inhibits the self-renewal of mouse embryonic stem cells
Photoactivation inhibits the proliferation of ChR2-GFP-V6.5 ESCs. a Cell proliferation assay of V6.5 ESCs and ChR2-GFP-V6.5 ESCs in both the photoactivated group and the nonphotoactivated control group. b, c Size and weight analysis of the teratoma formed by ChR2-GFP-V6.5 ESCs in both the photoactivated group and the nonphotoactivated control group. d, e Flow cytometric analysis of the cell cycle distribution of ChR2-GFP-V6.5 ESCs. (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001 compared to the nonphotoactivated control). f Immunostaining and semi-quantification assay of Ki67 expression in both the photoactivated group and the nonphotoactivated control group. (n = 6, *P < 0.05 compared to nonphotoactivated control)