Fig. 4

Intracellular localization of rFVII-160R after treatment with 4-PBA. Cells transiently expressing rFVIIwt or rFVII-160R were treated with 4-PBA for 42 h. A Confocal images from control cells or cells treated with 10 mM 4-PBA stained with FVII (green, a, d, g, j) and GM130 (red, b, e, h, k). Co-localized green and red pixels are shown in yellow color (c, f, i, l). B Confocal images from control cells or cells treated with 10 mM 4-PBA stained with FVII (green, a, d, g, j) and GRASP55 (red, b, e, h, k). Co-localized green and red pixels are shown in yellow color (c, f, i, l). Three independent experiments were performed. Bar 5 µm