Table 1 Primers for real-time RT-PCR analysis
ID | Sequence (5′–3′) | Product length (bp) |
|---|---|---|
Mouse | ||
β-actin F | CATTGCTGACAGGATGCAGA | 139 |
β-actin R | CTGCTGGAAGGTGGACAGTGA | |
PI3K F | ACACCACGGTTTGGACTATGG | 140 |
PI3K R | GGCTACAGTAGTGGGCTTGG | |
AKT F | AGAAGAGACGATGGACTTCCG | 111 |
AKT R | TCAAACTCGTTCATGGTCACAC | |
mTOR F | CACCAGAATTGGCAGATTTGC | 82 |
mTOR R | CTTGGACGCCATTTCCATGAC | |
LC3B F | CGCTTGCAGCTCAATGCTAAC | 93 |
LC3B R | CTCGTACACTTCGGAGATGGG | |
Beclin1 F | ATGGAGGGGTCTAAGGCGTC | 149 |
Beclin1 R | TGGGCTGTGGTAAGTAATGGA | |
Rat | ||
GAPDH F | CCTCGTCTCATAGACAAGATGGT | 169 |
GAPDH R | GGGTAGAGTCATACTGGAACATG | |
PI3K F | TTAACCCCCTCACAGCAGAG | 251 |
PI3K R | TTTCTGAACTGCAATGGCCC | |
AKT F | GCTGCTCAAGAAGGACCCTA | 218 |
AKT R | CTTGATCAGGCGGTGTGATG | |
mTOR F | GTTTGAAGTGAAGCGAGCCT | 231 |
mTOR R | CGCTGCGTGGTAAGAATCAG | |
LC3B F | TACCAAGGCAAAAAGGGACG | 282 |
LC3B R | CCCCTGACACTGCTCTTCTAT | |
Beclin1 F | GACCGAGTGACCATTCAGGA | 200 |
Beclin1 R | TGGTCTTCACAGGGTGCTAC | |