Fig. 2

SRC-3 activates Akt signaling to inhibit HBV gene expression. a Knockdown of SRC-3 decreased phosph-Akt expression in HepG2 cells. The expression of p-Akt and t-Akt were analyzed by western bolt. β-actin was served as a loading control. b–d Ectopic expression of constitutively active Akt (CA-Akt) reduced the mRNA levels of HBV and the protein levels of HBsAg and HBeAg. SRC-3-knockdown or control HepG2 cells were transfected with CA-Akt and pHBV1.3 plasmids for 48 h, and then the protein levels of SRC-3, p-Akt and t-Akt were detected by western blot (b), the mRNA level of HBV was quantified by real-time PCR (e), and the protein levels of HBsAg and HBeAg was measured by ELISA (d). All experiments were repeated at least 3 times independently. **p < 0.01, ***p < 0.001