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Fig. 2 | Cell & Bioscience

Fig. 2

From: Steroid receptor coactivator 3 inhibits hepatitis B virus gene expression through activating Akt signaling to prevent HNF4α nuclear translocation

Fig. 2

SRC-3 activates Akt signaling to inhibit HBV gene expression. a Knockdown of SRC-3 decreased phosph-Akt expression in HepG2 cells. The expression of p-Akt and t-Akt were analyzed by western bolt. β-actin was served as a loading control. bd Ectopic expression of constitutively active Akt (CA-Akt) reduced the mRNA levels of HBV and the protein levels of HBsAg and HBeAg. SRC-3-knockdown or control HepG2 cells were transfected with CA-Akt and pHBV1.3 plasmids for 48 h, and then the protein levels of SRC-3, p-Akt and t-Akt were detected by western blot (b), the mRNA level of HBV was quantified by real-time PCR (e), and the protein levels of HBsAg and HBeAg was measured by ELISA (d). All experiments were repeated at least 3 times independently. **p < 0.01, ***p < 0.001

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