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Fig. 1 | Cell & Bioscience

Fig. 1

From: Steroid receptor coactivator 3 inhibits hepatitis B virus gene expression through activating Akt signaling to prevent HNF4α nuclear translocation

Fig. 1

SRC-3 inhibits HBV biosynthesis in HepG2 cells. ac Knockdown of SRC-3 increased the mRNA levels of HBV and the protein levels of HBsAg and HBeAg. Stable SRC-3-knockdown and control HepG2 cells were transfected with pHBV1.3 plasmids, respectively. The SRC-3 protein was tested by western blot (a), the mRNA level of HBV was quantified by real-time PCR (b), and the protein of HBsAg and HBeAg was measured by ELISA (c). df Ectopic expression of SRC-3 in SRC-3-knockdown HepG2 cells reduced the mRNA levels of HBV and the protein levels of HBsAg and HBeAg. Stable SRC-3-knockdown HepG2 cells were transfected with SRC-3/pHBV1.3 plasmids or control/pHBV1.3 plasmids for 48 h. The protein levels of SRC-3 were detected by western blot (d), the mRNA level of HBV was quantified by real-time PCR (e), and the protein of HBsAg and HBeAg was measured by ELISA (f). All experiments were repeated at least 3 times independently. *p < 0.05, ***p < 0.001

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