Fig. 3

Modulation of PAF-induced IL-6 promoter activity by 48 kDa PTPN2 depends on multiple signaling pathways. HEK-PAFR were transiently co-transfected with the hIL-6-luc, control vector (pcDNA3), or the 48 kDa or 45 kDa PTPN2 constructs. Cells were incubated overnight in DMEM-0.2% BSA and a–e pre-treated for 30 min in DMEM-0.2%BSA with DMSO as vehicle control or a MEK 1/2 inhibitor U-0126, 5 µM, b JNK inhibitor SP600125, 1 µM, c pan PKC inhibitor GF109203x 2 µM or d Src kinase family inhibitor PP2, 10 nM and e Jak2 inhibitor AG490 5 µM. Stimulation with PAF (100 nM) or vehicle was for 6 h and luciferase activity was then measured. The data presented are a mean ± SEM of at least 4 independent experiments performed in triplicate. Significance was established with two-way ANOVA with Sidak post-test. *p < 0.05, **p < 0.01