Fig. 5

PinX1/NPM does not localize at Cajal Bodies at the start of S Phase. HeLa cells were synchronized at G1/S boundary by 2 mM hydroxyurea and were fixed at indicated time points. Immunofluorescence analysis showing localization of a NPM and coilin; and b PinX1 and coilin, at indicated stages of cell cycle after release from hydroxyurea block with anti-coilin (green) and anti-NPM (red) in (A); anti-PinX1 (red) in (B), and DAPI (blue) shows nucleus staining