Fig. 3
From: Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
crRNA competition effect during multi-gene KO. a U2OS-EGFP12-Cas9 cells were reverse transfected with two different KRAS crRNAs (KRAS#5 and KRAS#6) either alone or in combination with EGFP and PTEN crRNAs. Protein expression was assessed by western blot 5Â days post-transfection. b Quantification of target gene KO efficiency. Protein levels from a were quantified by densitometry. Parallel samples were collected on day 5 for target site mutation analysis using TIDE. The sample order in the bar chart corresponds to that of the western blot in a