Fig. 4

Functional analysis of the restored SLC26A3 function in reverted cells. a Sanger sequencing for confirming the correction of P131R SNP. We used a novel ssODN that codes for the same amino acids of WT SLC26A3, but uses unique codons for the F-P-I triAA sequence flanking the wild-type Proline that was changed to Arginine and now back to Proline. b TEER data from ECIS analysis of wild-type Caco-2 cells, Caco-2 cells containing the P131R and corrected cells (RWT). c Results from each group are presented as mean ± SD of three samples from three separate experiments. When SLC26A3-P131R was reversed back to wild type with the corrective donor templates by delivering Cas9/sgRNA vectors, a similar TEER and epithelial barrier function was observed in reverted cells. *P < 0.05, **P < 0.01, ***P < 0.001