Impact of SNPs interplay across the locus of MBL2, between MBL and Dectin-1 gene, on women’s risk of developing recurrent vulvovaginal infections

Table 2 PCR-RFLP protocol for the genotyping of MBL2 additional 5’ variants, restriction digestion conditions and expected restriction fragments size for MBL2 variants

MBL2 SNP	Enzyme	Restriction site	RFLP conditions	Wild-type homozygotes (bp)	Variant homozygotes (bp)	Heterozygotes (bp)
rs11003124	TspRI	5′…NNCASTGNN^▼…3′ 3′…_▲NNGTSACNN…5′	22 h at 65 °C	419 + 105	244 + 175 + 105	419 + 244 + 175 + 105
rs7084554	BanII	5′…GRGCY^▼C…3′ 3′…C_▲YCGRG…5′	4 h at 37 °C	551	357 + 194	551 + 357 + 194
rs36014597	NLaIII	5′…CATG^▼…3′ 3′…_▲GTAC…5′	4 h at 37 °C	308 + 155 + 122	430 + 155	430 + 308 + 155 + 122
rs11003123	BseYI	5′…C^▼CCAGC…3′ 3′…GGGTC_▲G…5′	4 h at 37 °C	322 + 184	506	506 + 322 + 184

Single letter code of restriction sites: R = A or G, Y = C or T, N = A or C or G or T, S = C or G

ISSN: 2045-3701