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Table 2 PCR-RFLP protocol for the genotyping of MBL2 additional 5’ variants, restriction digestion conditions and expected restriction fragments size for MBL2 variants

From: Impact of SNPs interplay across the locus of MBL2, between MBL and Dectin-1 gene, on women’s risk of developing recurrent vulvovaginal infections

MBL2 SNP Enzyme Restriction site RFLP conditions Wild-type homozygotes (bp) Variant homozygotes (bp) Heterozygotes (bp)
rs11003124 TspRI 5′…NNCASTGNN…3′
3′…NNGTSACNN…5′
22 h at 65 °C 419 + 105 244 + 175 + 105 419 + 244 + 175 + 105
rs7084554 BanII 5′…GRGCYC…3′
3′…CYCGRG…5′
4 h at 37 °C 551 357 + 194 551 + 357 + 194
rs36014597 NLaIII 5′…CATG…3′
3′…GTAC…5′
4 h at 37 °C 308 + 155 + 122 430 + 155 430 + 308 + 155 + 122
rs11003123 BseYI 5′…CCCAGC…3′
3′…GGGTCG…5′
4 h at 37 °C 322 + 184 506 506 + 322 + 184
  1. Single letter code of restriction sites: R = A or G, Y = C or T, N = A or C or G or T, S = C or G