Impact of SNPs interplay across the locus of MBL2, between MBL and Dectin-1 gene, on women’s risk of developing recurrent vulvovaginal infections

Table 1 PCR-RFLP protocol for the genotyping of MBL2 additional 5′ variants, PCR primers and conditions

MBL2 SNPs	Primer type	Primer sequence	Working primer conc. (p mol/µl)	PCR Conditions for all reactions	Product size (bp)
rs11003124^a	Forward	5′-GCTGGCTTATGCCTGTTAGC-3′	0.05	95 °C for 5 min, followed by 35 cycles (94 °C for 30 s, 53 °C for 30 s, and 72 °C for 45 s) and a final elongation at 72 °C for 5 min	524
rs11003124^a	Reverse	5′-CTGCTGAGGTTTCTTAGGGGG-3′	0.05		524
rs7084554^a	Forward	5′-AAGGAGGGGTTCATCTGTGC-3′	0.15		551
rs7084554^a	Reverse	5′-TGGGAGGAGGATTCAAGGCA-3′	0.15		551
rs36014597^b	Forward	5′-TCCTGCCAGAAAGTAGAGAGGT-3′	0.15		585
rs36014597^b	Reverse	5′-TGGCCTCTAGCTGGGGATTT-3′	0.15		585
rs11003123^a	Forward	5′-CTGGTTCCCCCTTTTCTCCC-3′	0.10		506
rs11003123^a	Reverse	5′-TGCACGGTCCCATTTGTTCT-3′	0.10		506

ISSN: 2045-3701