Skip to main content

Table 1 PCR-RFLP protocol for the genotyping of MBL2 additional 5′ variants, PCR primers and conditions

From: Impact of SNPs interplay across the locus of MBL2, between MBL and Dectin-1 gene, on women’s risk of developing recurrent vulvovaginal infections

MBL2 SNPs Primer type Primer sequence Working primer conc. (p mol/µl) PCR Conditions for all reactions Product size (bp)
rs11003124a Forward 5′-GCTGGCTTATGCCTGTTAGC-3′ 0.05 95 °C for 5 min, followed by 35 cycles (94 °C for 30 s, 53 °C for 30 s, and 72 °C for 45 s) and a final elongation at 72 °C for 5 min 524
Reverse 5′-CTGCTGAGGTTTCTTAGGGGG-3′ 0.05
rs7084554a Forward 5′-AAGGAGGGGTTCATCTGTGC-3′ 0.15 551
Reverse 5′-TGGGAGGAGGATTCAAGGCA-3′ 0.15
rs36014597b Forward 5′-TCCTGCCAGAAAGTAGAGAGGT-3′ 0.15 585
Reverse 5′-TGGCCTCTAGCTGGGGATTT-3′ 0.15
rs11003123a Forward 5′-CTGGTTCCCCCTTTTCTCCC-3′ 0.10 506
Reverse 5′-TGCACGGTCCCATTTGTTCT-3′ 0.10
  1. aDenote SNPs reported at forward strand
  2. bDenote SNPs reported at Reverse strand