Fig. 5
From: Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis
The subnuclear localization of Ezh2 alters from the nuclear periphery to interior nuclear after Emerin is knockdown in C2C12 myoblasts. a IF assays using C2C12 myoblasts with exogenous Flag-Msx1. Emerin was knockdown in C2C12 myoblasts using epiCRISPR/Cas9 system. Ezh2 was no longer localized at the nuclear periphery after Emerin was knockdown in C2C12 myoblasts. b IF assays using 293T cells with exogenous Flag-Msx1. Exogenous Msx1 was localized at the nuclear periphery in 293T cells, but Ezh2 distributed at the interior nuclear. Scale bars represented 10 µm