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Fig. 5 | Cell & Bioscience

Fig. 5

From: Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis

Fig. 5

The subnuclear localization of Ezh2 alters from the nuclear periphery to interior nuclear after Emerin is knockdown in C2C12 myoblasts. a IF assays using C2C12 myoblasts with exogenous Flag-Msx1. Emerin was knockdown in C2C12 myoblasts using epiCRISPR/Cas9 system. Ezh2 was no longer localized at the nuclear periphery after Emerin was knockdown in C2C12 myoblasts. b IF assays using 293T cells with exogenous Flag-Msx1. Exogenous Msx1 was localized at the nuclear periphery in 293T cells, but Ezh2 distributed at the interior nuclear. Scale bars represented 10 µm

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