Fig. 7
![Fig. 7](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13578-019-0291-1/MediaObjects/13578_2019_291_Fig7_HTML.png)
Phagocytic activity of G. mellonella hemocytes 24 h after topical application of norharman and harman. a Immunofluorescence localization of phagocytized E. coli bioparticles in hemocytes. β—actin (orange) was stained by ActinRed™ 555 ReadyProbes Reagent. Cell nuclei (blue) were stained with Hoechst. To visualize the phagocytosis of hemocytes the Escherichia coli (K-12 strain) BioParticles fluorescein (green) conjugate (Invitrogen) were used. b Relative phagocytosis. The data are expressed as the percentage of phagocytosis compared with control values (100%). Test was performed in three independent replicates; n = 20. 1N—norharman 750 ppm, 2N—norharman 1000 ppm, 3N—norharman 1250 ppm, 1H—harman 750 ppm, 2H—harman 1000 ppm, 3H—harman 1250 ppm; scale bar 25 µm