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Fig. 6 | Cell & Bioscience

Fig. 6

From: Harman and norharman, metabolites of the entomopathogenic fungus Conidiobolus coronatus (Entomophthorales), affect the serotonin levels and phagocytic activity of hemocytes, insect immunocompetent cells, in Galleria mellonella (Lepidoptera)

Fig. 6

Phagocytic activity of G. mellonella hemocytes 1 h after topical application of norharman and harman. a Immunofluorescence localization of phagocytized E. coli bioparticles in hemocytes. β-Actin (orange) was stained by ActinRed™ 555 ReadyProbes Reagent. Cell nuclei (blue) were stained with Hoechst. To visualize the phagocytosis of hemocytes the Escherichia coli (K-12 strain) BioParticles fluorescein (green) conjugate (Invitrogen) were used. b Relative phagocytosis. The data are expressed as the percentage of phagocytosis compared with control values (100%). Test was performed in three independent replicates; n = 20. 1N—norharman 750 ppm, 2N—norharman 1000 ppm, 3N—norharman 1250 ppm, 1H—harman 750 ppm, 2H—harman 1000 ppm, 3H—harman 1250 ppm; ***p ≤ 0.001; scale bar 25 µm

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Cell & Bioscience

ISSN: 2045-3701

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