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Fig. 10 | Cell & Bioscience

Fig. 10

From: Harman and norharman, metabolites of the entomopathogenic fungus Conidiobolus coronatus (Entomophthorales), affect the serotonin levels and phagocytic activity of hemocytes, insect immunocompetent cells, in Galleria mellonella (Lepidoptera)

Fig. 10

Phagocytic activity of G. mellonella hemocytes in vitro 24 h after norharman and harman treatment. a Immunofluorescence localization of phagocytized E. coli bioparticles in hemocytes. β-Actin (orange) was stained by ActinRed™ 555 ReadyProbes Reagent. Cell nuclei (blue) were stained with Hoechst. To visualize the phagocytosis of hemocytes the Escherichia coli (K-12 strain) BioParticles fluorescein (green) conjugate (Invitrogen) were used. b Relative phagocytosis. The data are expressed as the percentage of phagocytosis compared with control values (100%). Test was performed in three independent replicates; n = 20. 1N—norharman 750 ppm, 2N—norharman 1000 ppm, 3N—norharman 1250 ppm, 1H—harman 750 ppm, 2H—harman 1000 ppm, 3H—harman 1250 ppm; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; scale bar 25 µm

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Cell & Bioscience

ISSN: 2045-3701

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