Fig. 3

BLACAT1 promoted breast cancer cell survival via miR-150-5p. a, b MTT assay showed that cell proliferation was dramatically inhibited by knockdown of BLACAT1 or up-regulation of miR-150-5p in SKBR3 and MDA-MB-231 cells. c, d SKBR3 and MDA-MB-231 cell survival abilities were assayed by colony formation. SKBR3 and MDA-MB-231 cells were transfected with BLACAT1 siRNA or miR-150-5p mimics for 24 h, seeded in the 6-well plates culturing for 2 weeks and colonies were counted. e, f SKBR3 and MDA-MB-231 cell migration was assayed by wound-healing assay. SKBR3 and MDA-MB-231 cells were transfected with BLACAT1 siRNA or miR-150-5p mimics for 24 h and cell migration was analyzed. g, h SKBR3 and MDA-MB-231 cell migration was assayed by invasion assay. SKBR3 and MDA-MB-231 cells were transfected with BLACAT1 siRNA or miR-150-5p mimics for 24 h and cell invasion was analyzed