Skip to main content

Advertisement

Fig. 2 | Cell & Bioscience

Fig. 2

From: TUG1 confers cisplatin resistance in esophageal squamous cell carcinoma by epigenetically suppressing PDCD4 expression via EZH2

Fig. 2

TUG1 knockdown overcame DDP resistance of ESCC cells. a, b The cell viability was determined by MTT assay in ECA109/DDP and EC9706/DDP cells and their parental cells exposed to different concentrations of DDP (0.1, 1, 5, 10, 20, 40, 80, 160 μM) for 48 h. c, d qRT-PCR analysis was performed in ECA109/DDP and EC9706/DDP cells transfected with TUG1 siRNAs (si-TUG1 #1 or si-TUG1 #2) or si-con. e, f ECA109/DDP and EC9706/DDP cells transfected with siRNAs (si-TUG1 #1 or si-TUG1 #2) or si-con were treated with various concentrations of DDP (0.1, 1, 5, 10, 20, 40, 80, 160 μM) for 48 h and cell viability was evaluated by MTT assay. g, h Cell apoptosis was determined by flow cytometry analysis in siRNAs (si-TUG1 #1 or si-TUG1 #2) or si-con transfected ECA109/DDP and EC9706/DDP cells after treatment with 20 μM of DDP. *P < 0.05

Back to article page