Fig. 6
From: (+)-JQ1 attenuated LPS-induced microglial inflammation via MAPK/NFκB signaling
Anti-neuroinflmmation of (+)-JQ1 depends on MAPK/NFκB signaling in vivo. Mice were IVC injected with LPS (2 μg in 2 μl), together with or without (+)-JQ1 (50 mg/kg). 4 h later, mice were sacrificed and brain tissue samples were collected. The phosphorylation of p38, ERK1/2, JNK (a) and Akt (b) as well as the total protein content, was analyzed by immunoblot. Phosphorylation of IKKα/β (p-IKKα/β), IκBα (p-IκBα) and p65 (p-p65), as well as the total protein content, were analyzed by immnoblot (c). Representative results from three independent experiments are depicted here. The relative intensity of a given band was normalized. The data are presented as the mean ± SEM (n = 6)