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Fig. 4 | Cell & Bioscience

Fig. 4

From: (+)-JQ1 attenuated LPS-induced microglial inflammation via MAPK/NFκB signaling

Fig. 4

MAPK but not PI3 K signaling is targeted by (+)-JQ1. BV2 cells were pretreated with either (+)-JQ1 (400 nM) or DMSO for 30 min and subjected to LPS stimulation. The phosphorylation of ERK, p38, JNK (a) and Akt (b) as well as the total protein content, was analyzed by immunoblot. The relative intensity of a given band was normalized. Representative results from three independent experiments are depicted here. The relative intensity of a given band was normalized (n = 6). c BV2 cells were treated with LPS (100 ng/ml) or JQ1 (400 nM), together with or without U0126 (ERK inhibitor) or SB203580 (p38 inhibitor). 24 h later, cell culture medium were collected and IL-1β, IL-6 and TNFα were tested by ELISA (n = 3). The data are presented as the mean ± SEM (* p ≤ 0.05, ** p ≤ 0.01)

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