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Fig. 5 | Cell & Bioscience

Fig. 5

From: Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer

Fig. 5

Inhibitory effects of pantoprazole on STAT3 activity, cellular migration, and invasion are attenuated by silencing of SHP-1. a Western blot after transfection and 48 h of treatment with pantoprazole at 160 μg/ml. b Matrigel invasion assay. Upper panel: representative images assay. Black bar indicates 50 μm. Lower panel: Comparison of invading cells. Data are presented as mean ± standard deviation. Cell counting was performed in five randomly selected separate areas under ×20 magnification. *P < 0.05, compared with pantoprazole 160 μg/ml + siControl (n = 5). c Wound closure assay. Upper panel: representative images assay. Black bar indicates 200 μm. Lower panel: Comparison of vertical wound distance. Data are presented as mean ± standard deviation. All experiments were performed in triplicates. *P < 0.05, compared with pantoprazole 160 μg/ml + siControl (n = 3). d 3-D culture spheroid cell invasion assay. Upper panel: representative images of 3-D culture spheroid invasion assay. Black bar indicates 200 μm. Lower panel: comparison of area of spheroids. Data are presented as mean ± standard deviation. All experiments were performed in triplicates. *P < 0.05, compared with control; #P < 0.05, compared with pantoprazole at 40 μg/ml (n = 3). PPZ, pantoprazole

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