Fig. 2

TIPE2 overexpression markedly suppressed the viability and proliferation of PTC cells. A-B. Real-time PCR (a) and western-blot (b) showed that TIPE2 expression was significantly upregulated after transfection of the PRK5-TIPE2 plasmid in TPC-1 and B-CPAP cells. c, d After TIPE2 overexpression, CCK8 assays were conducted to evaluate the viability of TPC-1 (c) and B-CPAP cells (d). e, f MTT assays were conducted to evaluate the proliferation of TPC-1 (e) and B-CPAP cells (f) after TIPE2 overexpression. Data represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001