Table 1 Primers, amplicon size and annealing temperature of Tf, TfR, Ferritin, IRP1 and β-actin for RT-PCR
From: Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington’s disease
Name | Primer sequences | Amplicon size (bp) | Annealing temperature (°C) | |
|---|---|---|---|---|
Forward | Reverse | |||
β-actin | GTCGTACCACAGGCATTGTGATGG | GCAATGCCTGGGTACATGGTGG | 492 | 58/63 |
β-actin | TTTCCAGCCTTCCTTCTTGGGTATG | ATAGAGGTCTTTACGGATGTCAACG | 100 | 58/63 |
Tf | AAACGGTCAAATGGTGCG | CTCCGACAGCTTACAGAAGAGC | 413 | 58 |
TfR | TCCCGAGGGTTATGTGGC | GGCGGAAACTGAGTATGATTGA | 324 | 58 |
Ferritin | TTTGACCGAGATGATGTG | TCAGTAGCCAGTTTGTGC | 248 | 58 |
IRP1 | GACATCGTGCTCACCATTACCAA | TGAAATCTCGAAACATGCCTACA | 265 | 58 |