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Fig. 3 | Cell & Bioscience

Fig. 3

From: RINCK-mediated monoubiquitination of cGAS promotes antiviral innate immune responses

Fig. 3

RINCK is required for cGAMP synthesis. a WT or RINCK-deficient U937 cells were treated with cGAMP for indicated time followed by measuring IFN-β mRNA with qPCR. b WT or RINCK-deficient U937 cells were treated with cGAMP for indicated time followed by immunoblotting with indicated antibodies. c Standard curve for cGAMP quantification by LC–MS/MRM. d WT or RINCK-deficient U937 cells were treated with HT-DNA for 6 h and the cell extract was collected for quantification of cGAMP by LC–MS/MRM. Data are presented as the mean ± SD. ***P < 0.001. N.D., not detected. Data represent three independent experiments

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