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Fig. 5 | Cell & Bioscience

Fig. 5

From: TP53TG1 enhances cisplatin sensitivity of non-small cell lung cancer cells through regulating miR-18a/PTEN axis

Fig. 5

TP53TG1 regulated PTEN expression through miR-18a in NSCLC cells. a PTEN expression was assessed by western blot in A549 cells transfected with miR-18a mimics or anti-miR-18a. b Western blot assay of PTEN expression in A549 cells transfected with si-TP53TG1#1 or pcDNA-TP53TG1. Dual-luciferase reporter assay was performed by transfecting PTEN-WT vector into A549 cells together with miR-18 mimics or miR-18a mimics + pcDNA-TP53TG1 (c), and anti-miR-18a or anti-miR-18a + si-TP53TG1#1 (d). e qRT-PCR assay of PTEN expression in 40 pairs of NSCLC tumor samples. f qRT-PCR assay of PTEN expression in DDP-sensitive NSCLC tissues and DDP-resistant NSCLC samples. g The correlation analysis between TP53TG1 and PTEN expression in NSCLC tumor specimen. Each experiment is repeated three times. *P < 0.05 vs. respective control

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