Fig. 6

AQP1 was a target of miR-144-3p in A549 cell. a According to the predicted binding site of miR-144-3p in AQP1 transcript, we constructed a mutant plasmid of AQP1. b The miR-144-3p inhibitor enhanced the luciferase activity in WT-AQP1-transfected A549 cell, while have no impact in MUT-AQP1-transfected A549 cell. Meanwhile, the AQP1 mRNA and protein expression levels were significantly increased in miR-144-3p inhibitor-transfected A549 cells. c The miR-144-3p mimic reduced the luciferase activity in WT-AQP1-transfected A549 cell, while have no impact in MUT-AQP1-transfected A549 cell. Meanwhile, the AQP1 mRNA and protein expression levels were significantly decreased in miR-144-3p mimic-transfected A549 cells. *P < 0.05 vs pre-NC, ^#P < 0.05 vs NC