Fig. 3

LPS induced the EMT of colon cancer cells via expression of TLR4. a, b Real-time PCR was employed to observe the effect of LPS on the EMT level of C26 and HCT116 cells. c Western blot was used to detect the expression of Vimentin and E-cadherin in C26 cells. d The inhibitory effect of siRNA on TLR4 expression in C26 cells was examined by western blot. e Transwell assay was used to detect the metastasis of C26 cells with TLR4 siRNA treatment. f, g Real-time PCR was employed to observe the EMT level of C26 cells when exposed to LPS with or without the expression of TLR4. (*P<0.05, **P < 0.01, ***P < 0.001)