Fig. 2

Over-expression of miR-16 promoted proliferation and inhibited apoptosis in IL-1β-induced chondrocytes. a The expression of miR-16 was assessed by RT-qPCR in cartilage tissues of rat OA model (n = 10) and sham group cartilage tissues (n = 10). b–e Chondrocytes were stimulated with 10 ng/ml IL-1β for 2 h, then transfected with miR-NC, miR-16 mimics, anti-miR-NC or anti-miR-16. b Relative miR-16 expression was detected in IL-1β-induced chondrocytes transfected for 48 h. c Cell viability of IL-1β-induced chondrocytes transfected for 0, 24, 48 and 72 h was determined by Cell Counting Kit-8 (CCK-8) at OD450 nm. d Cell apoptosis of IL-1β-induced chondrocytes transfected for 48 h was detected by flow cytometry using Annexin V-FITC Apoptosis Assay kit. e Bax and Bcl2 expression were detected in IL-1β-induced chondrocytes after 48 h transfection by western blot analysis. *P < 0.05 vs. negative control