Fig. 5

DLL4 knockdown promoted proliferation and neural differentiation in NSCs. NSCs were transfected with si-DLL4 or si-control and cultured for 48 h. a miR-124 expression in transfected NSCs was detected by qRT-PCR. b MTT assay was conducted to assess cell proliferation in transfected NSCs. The mRNA (c) and protein (d) levels of ki-67 in transfected NSCs were determined by western blot. e The mRNA expression of Nestin in transfected NSCs was analyzed by qRT-PCR. f The neurosphere number was counted. The mRNA and protein levels of β-tubulin-III (g, i) and GFAP (h, j) in transfected NSCs were examined by qRT-PCR and western blot. *P < 0.05, n = 3