Fig. 4

miR-124 overexpression promoted proliferation and neural differentiation in NSCs. NSCs were transfected with miR-124, anti-miR-124, or respective controls. a qRT-PCR analysis of miR-124 expression in transfected NSCs. b MTT assay was performed to detect cell viability at 0, 24, 48 and 72 h in transfected NSCs. The mRNA (c) and protein (d) levels of ki-67 in transfected NSCs were evaluated by qRT-PCR and western blot. e The mRNA expression of Nestin in transfected NSCs was measured by qRT-PCR. f The neurosphere number was counted. The mRNA and protein levels of β-tubulin-III (g, i) and GFAP (h, j) in transfected NSCs were evaluated by qRT-PCR and western blot. *P < 0.05, n = 3