Fig. 4

14-3-3ζ and miR-126 regulate endothelial migration and tube formation. a Transwell migration assay of mouse endothelial cells with 14-3-3ζ knockdown and rescue by overexpression of exogenous miR-126. Cells stably expressing scramble or 14-3-3ζ shRNA were transfected with control or pre-miR-126. Representative result is shown in the top panel and the bottom panel shows the quantification. Scale bar indicates 50 μm in length. b Tube formation assay with exogenously expressed miR-126 in the 14-3-3ζ knockdown mouse endothelial cells. Representative results are shown in the top panel and the bottom panel shows the quantification. Scale bar indicates 50 μm in length. c Mouse endothelial cells stably transfected with either scrambled shRNA or shRNA targeting 14-3-3ζ were transfected with pre-miR-126. MiR-126 expression was analyzed by qRT-PCR and normalized to PECAM1, ± SD (n = 3). Three independent transwell experiments were performed and three random fields from the tube formation assay were analyzed for statistical analysis