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Fig. 3 | Cell & Bioscience

Fig. 3

From: Sirtinol regulates the balance of Th17/Treg to prevent allograft rejection

Fig. 3

The effect of sirtinol on the proliferation and differentiation of T cells subset. a The recipient spleens were harvested 7 days after transplantation. CD3+ T cells, CD4+ T cells and CD8+ T cells from splenocyte were measured by flow cytometry. b The recipient spleens were harvested 7 days after transplantation. CD4+ T cells were isolated and CD25+ and Foxp3+ T cells were measured by flow cytometry. Shown are representative of five separate experiments. c The recipient spleens were harvested 7 days after transplantation. CD4+ T cells were isolated and IL-17A+ T cells were measured by flow cytometry. Shown are representative of five experiments. d Percentages of CD4+, CD25+Foxp3+ and IL-17A+ cells were presented. e ELISA assays were performed to test the IL-17A levels on day 7 after transplantation. The data are expressed as mean ± SD (n = 5). *P < 0.05, **P < 0.01, compared with DMSO group

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