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Table 2 Comparative quantification of eluted proteins obtained from each column condition

From: Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique

Condition of column Bound protein (µg ± SD)a Flow-through (µg ± SD)a % of eluted proteinb
Elution step using 0.1 M glycine–HCl pH 2.7
 B. pseudomallei WT 13.46 ± 1.09 8.30 ± 0.14 61.77 ± 2.89
 B. pseudomallei rpoS mutant 12.69 ± 1.63 8.65 ± 0.11 68.56 ± 7.15
 Negative controlc 5.00 ± 1.09 3.95 ± 0.11 89.82 ± 13.26
Washing step
 B. pseudomallei WT 13.46 ± 1.09 4.60 ± 0.28 35.66 ± 2.10
 B. pseudomallei rpoS mutant 12.69 ± 1.63 3.25 ± 0.18 26.59 ± 1.39
 Negative controlc 5.00 ± 1.09 ND ND
  1. aData was obtained from independent experimental replicate
  2. b% of eluted protein was calculated from eluted protein (μg) × 100/initial protein existed in column (μg)
  3. cNegative control represent to a column condition containing crude proteins without LC3 expression bait for protein of B. pseudomallei WT strain
  4. ND represent to no determination because protein concentration could not be detected based on Bradford assay