|
Condition of column
|
Bound protein (µg ± SD)a
|
Flow-through (µg ± SD)a
|
% of eluted proteinb
|
|---|
|
Elution step using 0.1 M glycine–HCl pH 2.7
|
|
B. pseudomallei WT
|
13.46 ± 1.09
|
8.30 ± 0.14
|
61.77 ± 2.89
|
|
B. pseudomallei rpoS mutant
|
12.69 ± 1.63
|
8.65 ± 0.11
|
68.56 ± 7.15
|
|
Negative controlc
|
5.00 ± 1.09
|
3.95 ± 0.11
|
89.82 ± 13.26
|
|
Washing step
|
|
B. pseudomallei WT
|
13.46 ± 1.09
|
4.60 ± 0.28
|
35.66 ± 2.10
|
|
B. pseudomallei rpoS mutant
|
12.69 ± 1.63
|
3.25 ± 0.18
|
26.59 ± 1.39
|
|
Negative controlc
|
5.00 ± 1.09
|
ND
|
ND
|
-
aData was obtained from independent experimental replicate
-
b% of eluted protein was calculated from eluted protein (μg) × 100/initial protein existed in column (μg)
-
cNegative control represent to a column condition containing crude proteins without LC3 expression bait for protein of B. pseudomallei WT strain
- ND represent to no determination because protein concentration could not be detected based on Bradford assay
