Fig. 5

BMPR1b expression in developing oocytes was reduced in SENP1-smKO mice. Ovaries from WT and SENP-smKO mice were harvested at postnatal day 7. a Protein levels of BMP4 and receptors were determined by western blot with respective antibodies. Normalized protein levels (with GADPH) are presented as fold changes by taking WT group as 1.0. n = 3. b BMP4 and SM22α co-immunofluorescence staining of ovaries from WT and SENP-smKO mice. c BMP4 and BMPR2 co-immunofluorescence staining of ovaries from WT and SENP-smKO mice. d, e SM22α and BMPR1B or BMPR2 co-immunofluorescence staining of ovaries from WT and SENP-smKO mice at postnatal day 7. Scale bars 15 μm. OV ovary, OC oocyte, GC granulosa cell, SC stromal cell. Asterisks indicate the same oocyte in the merged panel