Fig. 3

Stromal SENP1 deletion delays early follicle development in neonatal mouse. a–c Stromal SENP1 deletion reduces follicle size and number in neonatal mice. Hematoxylin and Eosin staining of ovaries from WT and SENP-smKO mice at postnatal day 3 (a) and day 7 (b). Scale bars 160 μm. Sizes of primary and secondary follicles in ovaries of WT and SENP1-smKO mice are quantified (c). Data are presented as mean ± SEM, n = 5, *P < 0.05, **P < 0.01. d–f Stromal SENP1 deletion delays early folliculogenesis in neonatal mice. VASA immunofluorescence staining of ovaries from WT and SENP-smKO mice at postnatal day 3 (d) and day 7 (e). Scale bars 60 μm in ×10 images; 15 μm in ×40 images. Number of primary and secondary follicles are quantified (f). Data are presented as mean ± SEM, n = 5, **P < 0.01. OV ovary, PrF primary follicle, ScF secondary follicle