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Fig. 5 | Cell & Bioscience

Fig. 5

From: Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Fig. 5

Epigenetic regulation of the Runx2 promoter in Nampt deficient MC3T3-E1 cells. a ChIp-PCR of Runx2 promoter following immunoprecipitation with the Acetyl Histone H3 (K9) antibody in scrambled shRNA (scRNA) or Nampt shRNA transduced stable MC3T3-E1 clones with or without differentiation for 72 h. b Densitometry analysis of differentiation (OBM)-mediated Runx2 promoter acetylation normalized to input and undifferentiated cells transduced with scrambled shRNA. Bars are mean ± SD. Each experiment performed in triplicate. *p < 0.05

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