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Fig. 1 | Cell & Bioscience

Fig. 1

From: Small GTPase Arl6 controls RH30 rhabdomyosarcoma cell growth through ciliogenesis and Hedgehog signaling

Fig. 1

Arl6 regulates ciliogenesis and Hedgehog signaling transduction at the base of primary cilium. a Confocal images showing distribution of exogenously expressed Arl6-RFP (red) or its T31R, Q73L mutants in MEFs. Arl6-RFP and its Q73L mutant accumulated in basal body of primary cilium (green), but T31R did not. b Western blot showing the same expression level of Arl6-RFP and its mutants in MEFs. β-actin was used as a loading control. c Representative confocal images of anti-acetylated tubulin (green) showing the lack of primary cilium in stable Arl6−/− MEFs compared to WT. Nucleus are stained with DAPI (blue). d Quantitation of percentage (left) and ciliary length (right) of ciliated cells from c. e RT-PCR detection of Gli1, Arl6 in WT and Arl6−/− MEFs stimulated with Shh conditional medium. HPRT mRNA was used as an internal control. A representative gel image is shown here. The relative intensity of the bands is shown in Additional file 1: Figure S1. f Q-PCR quantification of fold induction of Gli1 mRNA from an experiment as in e. Fold induction was calculated using Gli1 mRNA level normalized against that of HPRT for even loading and then against the normalized Gli1 mRNA level from WT cells without Shh treatment. The results represent the mean ± standard error of the mean (SEM) of three independent experiments *P < 0.05, ***P < 0.001 vs. Arl6+/+ control

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