Skip to main content

Advertisement

Fig. 3 | Cell & Bioscience

Fig. 3

From: Targeted genome engineering in Caenorhabditis elegans

Fig. 3

The figure was adapted from Arribere et al. [51]

The co-CRISPR and co-conversion strategies for the detection of targeted genome modifications. a The co-CRISPR strategy used rol-6(su1006) expression plasmid as a co-injection marker and an unc-22 sgRNA as a co-editing marker. F1 animals with both twitching and rolling phenotypes are selected. The twitching F2 animals are further screened by single worm PCR to identify the animals with gene X mutation. b The co-conversion strategy used a donor oligonucleotides carrying the rol-6(su1006) mutation as both co-injection and editing marker. F1 roller animals are screened by single worm PCR to identify the animals with gene X mutation.

Back to article page