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Fig. 5 | Cell & Bioscience

Fig. 5

From: Downregulation of long non-coding RNA H19 promotes P19CL6 cells proliferation and inhibits apoptosis during late-stage cardiac differentiation via miR-19b-modulated Sox6

Fig. 5

The effect of Sox6 knockdown and overexpression on P19CL6 cell proliferation and apoptosis. a CCK-8 assay showed that pcDNA-Sox6 significantly inhibited cell viability at day 8 and 10, and si-Sox6 significantly promoted cell viability at day 8 and 10. b Cell cycle analysis found that pcDNA-Sox6 significantly decreased the cell number in S phase at day 8 and 10, and si-Sox6 obviously increased the cell number. c Flow cytometry showed that si-Sox6 significantly reduced the percentage of apoptotic cells from day 6 to 10. However, pcDNA-Sox6 played an opposite role in apoptosis at day 8 and 10. d Activity of caspase-3 was significantly reduced in cells transfected with si-Sox6 and significantly enhanced in cells transfected with pcDNA-Sox6. *P < 0.05, **P < 0.01, ***P < 0.001

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