Fig. 2

The effects of different treatments on the proliferation of endothelial cells (ECs). A The ECs treated with the indicated reagents for 24 h were subjected to BrdU staining (×200), and the number of positive ECs from at least five random fields per group were counted. Representative images are shown for the a PBS control; b VEGF; c–e 0.1, 1.0 and 10.0 µM roxarsone; f LY294002 and g LY294002 plus 1.0 µM roxarsone groups. B The number of positive cells are presented as the mean ± SD values from three independent experiments. *p < 0.05, **p < 0.01 vs. the PBS control; ^##p < 0.01 vs. 1.0 µM roxarsone