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Fig. 4 | Cell & Bioscience

Fig. 4

From: Hedgehog pathway is involved in nitidine chloride induced inhibition of epithelial-mesenchymal transition and cancer stem cells-like properties in breast cancer cells

Fig. 4

NC inhibited Hedgehog pathway in MDA-MB-468 and MCF-7 cell lines. Cells were treated with various concentrations of NC for indicated time. a The Gli1, Gli2, Smo and Patched1 proteins were examined by western blot in both cells. b Treatment of both cell lines with NC (0, 1.0, or 2.5 μM) for 48 h resulted in a significant inhibit the activation of Hedgehog signaling pathway in a dose-dependent manner, β-actin was used as a loading control. c Then, the expressions of Gli1 and Smo were determined by real-time PCR with GAPDH as a loading control. Results are presented as mean ± SD

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