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Fig. 6 | Cell & Bioscience

Fig. 6

From: Potential role of oxidative stress-induced apoptosis in mediating chromosomal rearrangements in nasopharyngeal carcinoma

Fig. 6

A map illustrating the positions of H2O2-induced chromosomal breaks within the AF9 gene. (a) The AF9 genomic map from nucleotide positions 601-281480 [EMBL:ENSG00000171843]. Black vertical lines show the locations of exons 1–10. Green boxes represent the two previously identified patient breakpoints cluster regions which are indicated as BCR1 and BCR2 [34]. Yellow boxes show the two previously experimentally isolated MAR/SAR which are indicated as SAR1 and SAR2 [34]. BamH I (B) restriction sites are shown. b The region of study (10.2 kb). BamH I (B), Kpn I (K) and Nde I (N) restriction sites are shown. Green arrows represent the primers (R1, AF9 236451 R and F1, AF9 245385 F) used in the first round of nested IPCR while blue arrows represent the primers (R2, AF9 236211 R and F2, AF9 245507 F) used in the second round of nested IPCR. (c) Breakpoints mapped in H2O2-treated cells. Green and red vertical lines represent the presently detected breakpoints in H2O2-treated HK1 and NP69 cells respectively. All the chromosomal breaks were mapped within BCR1 in close proximity with SAR1. Blue box represents the AF9 region (at coordinates 245252-245612) that translocates with the MLL gene, resulted in MLL-AF9 fusion gene previously identified in ALL patient [GenBank:AM050804]

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