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Fig. 7 | Cell & Bioscience

Fig. 7

From: Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway

Fig. 7

Exogenous H2S inhibited activation of AMPK through ameliorating mitochondrial injury induced by oxidative stress. a The mitochondrial CAT and SOD level were examined by Western blotting. Each OD value was standardized to that of VDAC. There data were summarized from at least three different experiments. (*p < 0.05 vs control group. **p < 0.01 vs control group. #p < 0.05 vs HG + Pal group). b The mRNA levels of SOD and CAT were examined by real-time PCR. (*p < 0.05 vs control group). c The expression of Nrf2 in the nucleus and in the cytoplasm was examined by Western blotting. The OD value of nuc-Nrf2 was standardized to that of histone3. The OD value of Cyt-Nrf2 was standardized to that of β-actin. There data were summarized from at least three different experiments. (##p < 0.01 vs HG + Pal group. ###p < 0.001 vs HG + Pal group)

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