Fig. 2

Critical roles of STAT5 and AKT in MPL W515L-induced hyperproliferation and compensating the loss function of MPL515/625/630. a MPL W515L- transduced G1ME cells were treated with DMSO or inhibitors as indicated. Cell numbers were counted and normalized to the starting cell numbers (Day 0) as proliferation rate. b MPL515/625/630-transduced G1ME cells were further infected with control GFP-expressing retrovirus or virus expressing GFP and proteins as indicated. The percentages of GFP⁺ in the resultant cells cultured with (solid round) or without TPO (hollow round) over time were analyzed by flow cytometry. *, **, *** indicates p < 0.05, 0.01, 0.001, respectively