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Fig. 3 | Cell & Bioscience

Fig. 3

From: Ammonium ions improve the survival of glutamine-starved hybridoma cells

Fig. 3

Ammonium salts improve the viability of glutamine-starved Sp2/0-Ag14 cells—clonogenic and flow cytometry assays. Cells were deprived of l-glutamine for 3 h in the presence of 5 mM ammonium salt/sodium salt or an equivalent volume of PBS. Panels a and b, clonogenic assay. Panel c, Annexin-V/propidium iodide assay. Cultures in which Gln was added at the start of the experiment were included as controls. *p < 0.05 vs corresponding sodium salt-supplemented control

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