Fig. 1
Paeoniflorin (PF) protects the heart against MI-induced injury in DM mice. a PF decreased myocardial infarct size. The black arrow indicates the infarction site. b PF decreased the release of myocardial enzymes in serum, including CK, CKMB, GOT, a-HBDH, and LDH. c the activity of CGRP in serum measured at different time points before and after MI. d Heart function was analyzed by measuring fractional shortening {FS = [LV end-diastolic diameter (LVEDD) − LVend-systolic diameter (LVESD)] × 100/LVEDD} and LV ejection fraction [LVEF = (LVEDD2 − LVESD2)/LVEDD2]. d Representative immunoblots of TRPV1 expression are shown. GAPDH was used as control. The lower panel shows the statistical analysis of the upper panel; *P < 0.05. f Representative images of hematoxylin and eosin (1–4) and Masson’s trichrome staining (5–8); (1 and 5) normal group (2 and 6) WTDM group (3 and 7) PF-WTDM-L group (4 and 8) PF-WTDM-H group. WTDM group: WTDM mice were pretreated with saline, PF-WTDM-L group: WTDM mice were pretreated with PF (70 mg/kg) before surgery, and PF-WTDM-H group: WTDM mice were pretreated with PF (140 mg/kg) before surgery (n = 6 per group; *P < 0.01, PF-WTDM-H vs. WTDM)