Fig. 2From: Restored expression of vitamin D receptor and sensitivity to 1,25-dihydroxyvitamin D3 in response to disrupted fusion FOP2–FGFR1 gene in acute myeloid leukemia cellsExpression of VDR gene and regulation of VDR and protein in AML cell lines in response to 1,25D and ATRA. KG1, HL60, KG1-CtrA and KG1-RARA cells were exposed to 10 nM 1,25D or to 500 nM ATRA or both for 72 h and then expression of VDR protein was studied (a). The cells were fractionated into cytosolic, nucleosolic and chromatin-bound fractions. Fractionated proteins were analyzed in western blots using anti-VDR, anti-actin and anti-Histone antibodies. The experiments were repeated from 2 (HL60) to 5 (KG1) times and representative blots are presented. C control, D 10 nM 1,25D; A 500 nM ATRA. The constitutive expression levels of VDR mRNA (b) in the above cell lines were measured by Real-time PCR relative to GAPDH expression levels. The bar charts show mean values of three experiments (±SEM) of relative quantity (RQ)Back to article page